RE: [Histonet] trichrome staining

From:"Bartlett, Jeanine \(CDC/CCID/NCZVED\)"

But with the One-Step you must post-mordant with Bouin's.
-----Original Message----- 
From: on behalf of Malcolm McCallum 
Sent: Tue 10/17/2006 4:08 PM 
To: White, Lori; 
Subject: RE: [Histonet] trichrome staining

	According to Humason's Animal Tissue Techniques (5th ed.), Pg 126.  You can use Masson Trichrome Staining method with any general fixative.  Pollak Rapid Method (pg 128) can also use any general fixative, although mercuric chloride will improve formalin fixed tissues.  Finally, you can use Gomori's one-step Trichrome as it is intended for use with neutral buffered formalin (pg 129).
	Hope that helps!
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	From: on behalf of White, Lori
	Sent: Tue 10/17/2006 2:03 PM
	Subject: [Histonet] trichrome staining
	Does anybody have a method for trichrome staining that does not use
	picric acid or mercury for fixation?  References would be wonderful if
	you have them.
	Lori White
	Peterborough, Ontario
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