RE: [Histonet] sections curling

From:"Tamara Franz-Odendaal"


I section decalcified bone in paraffin routinely. I cut 3-8um depending
on the tissue. Curling and wrinkling may have to do with humidity in the
room, we find this often.  Also try putting your block in the freezer
overnight if you don't already. I do not use disposable blades.

Hope this helps


Tamara Franz-Odendaal
Mount Saint Vincent University
Halifax, NS, Canada

Tamara Franz-Odendaal (PhD)
NSERC University Faculty Awardee
Biology Dept, 

Mount Saint Vincent University
166 Bedford Highway
Halifax, NS,  B3M 2J6

Tel: 902 - 457 6140
Fax: 902 - 457 6455

>>> "Kemlo Rogerson"  10/25/2006 4:45
AM >>>

sorry for the simplistic question.  We are having a lot of problem
some sections curling and wrinkling.


this is decalcified bone in parafin.  We are using a sliding microtome
with disposable blades.  


is it the blades?  This is my first use of disposables.  


It seems like the answer is very simple, but I have drawn a blank.  


Bet it's the processing, but it could be the angle of the blade.
your processing schedule and try altering the angle of attack for your
blade (what is the correct name for that angle?).



Kemlo Rogerson

Pathology Manager

Ext  3311

DD   01934 647057

Mob 07749 754194

Pager 07659 597107


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