RE: [Histonet] sections curling

From:"Kemlo Rogerson"


sorry for the simplistic question.  We are having a lot of problem with
some sections curling and wrinkling.


this is decalcified bone in parafin.  We are using a sliding microtome
with disposable blades.  


is it the blades?  This is my first use of disposables.  


It seems like the answer is very simple, but I have drawn a blank.  


Bet it's the processing, but it could be the angle of the blade. Revisit
your processing schedule and try altering the angle of attack for your
blade (what is the correct name for that angle?).



Kemlo Rogerson

Pathology Manager

Ext  3311

DD   01934 647057

Mob 07749 754194

Pager 07659 597107


Nobody grows old merely by living a number of years. We grow old by
deserting our ideals. Years may wrinkle the skin, but to give up
enthusiasm wrinkles the soul. --Samuel Ullman 



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