Last week we used a Mallory bleach to remove the melanin, which worked
well enough for Mart-1(Melan A) but not for HMB45.    We then use a VIP
purple DAB product ( from Vector) on another set of slides without the
bleach.   Whatever it takes

Dave
Christie Hosp, Manchester UK

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry,
Margaret
Sent: 27 October 2006 19:00
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] melanin pigment

Mel wrote: 

I don't recall this question, but I would be interested in how you get
around the problem of epidermal pigmentation if you are looking for
protein expression in the epidermis; do you bleach the tissue (and if
so, does that affect antigenicity of the proteins), or can you choose a
detection chromogen that has a wild color, like a Barney purple color? 

Thanks for your help.

Mel

 

We use Vectors Nova Red and it works well with melanin and also
hemosiderin.

 

Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007

 

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