RE: [Histonet] double staining

From:"Liz Chlipala"

If I remember correctly from Chris van der Loos lecture and his book on
multiple staining methods if the two antigens are co-localized then DAB is
not a suitable chromagen. If the antigens are not co-localized then DAB
would be fine.  Please feel free to correct me if I'm remembering this
wrong. I commonly use DAB with Fast Red for antigens that will stain
different cells or different cellular components.  


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Tyler
Sent: Wednesday, October 25, 2006 2:06 AM
To: histonet
Subject: [Histonet] double staining

Morning to All Histonetters
Request from one of the Med Techs I work with. Please can someone
forward or suggest a method for double staining on tissue sections using
DAB and AEC substrate.

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