RE: [Histonet] TRAP staining on undecalcified bone

From:"Patsy Ruegg"

Sigma still sells a kit for TRAP the enzyme histochemical reaction cat. No.
387-A  Leukocyte Acid Phosphatase this is a kit.

-----Original Message-----
[] On Behalf Of John A.
Sent: Wednesday, November 01, 2006 8:24 AM
To: Tiffany Pitts
Subject: Re: [Histonet] TRAP staining on undecalcified bone

Is TRAP is tartrate-resistant acid phosphatase (an
enzyme in osteoclasts; also in osteoblasts near
resorbing bone surfaces)? 

If so, try following up this internet link:

Unfortunately items from the
archives (Histonet questions and answers) are
undated, but none can be more than about 10 years
old! The author of the above link is a bone
expert; she edits the "Hard Times" NSH newsletter.

John Kiernan
Anatomy, UWO
London, Canada
Tiffany Pitts wrote:
> Hello all,
> I am trying to do TRAP staining on undecalcified mouse bones cut from
> Methylmethacrylate embedded blocks. My problem is that I cannot seem to
> a protocol that works well and/or is on the market any more. Does anyone
> know of a protocol I can use or (even better) a kit? Our in-house protocol
> is light on details and I'm not sure if I'm doing it right.
> Thank you
> Tiffany Pitts
> Department of Urology
> University of Washington
> Seattle, WA
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