RE: [Histonet] Melanin problem

From:"Liz Chlipala"


When I'm dealing with endogenous brown pigment I switch to a AP detection
system and use Fast Red or another red chromagen or if you want to stay with
a HRP system then use AEC.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of
Sent: Friday, October 27, 2006 8:40 AM
Subject: [Histonet] Melanin problem

I don't recall this question, but I would be interested in how you get 
around the problem of epidermal pigmentation if you are looking for 
protein expression in the epidermis; do you bleach the tissue (and if so, 
does that affect antigenicity of the proteins), or can you choose a 
detection chromogen that has a wild color, like a Barney purple color? 
Thanks for your help.
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
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