If you like the PTAH stain, instead of the mercuric post-fixative, place it
in Bouins in a 60 degree C. oven for 45-60 minutes, wash in running water
1-10 minutes until all the yellow is gone, and then stain as usual in the
PTAH. 

This PTAH stain was on our HTL exam when I was a student, and the sat.
mercuric chloride post-mordant was giving us splotchy stains. Same with
using Zenker as the post-mordant. One of my classmates (Liz) found the
Bouins post-mordant PTAH procedure in Ann Preace's book. We tried it, it
worked better than the mercuric chloride, and we've been using this method
at our hospital for the last 25 years.

Works every time, no splotchiness, no mercury, and, if you're already doing
trichromes, you already have the Bouins so there's nothing special to make
up.

If, on the other hand, you would like our lab's version of MSB (again, no
mercury, basically a trichrome with martius yellow add to stain RBCs
(quadrachrome?) - email me at my WORK email = pwenk@beaumont.edu  Do NOT
reply back to Histonet. That comes to my home, and the procedures are at
work.

MSB = collagen blue; RBC yellow; fibrin red (if young, yellow; if old,
bluish); nuclei black to red; all other cells red (muscle, cytoplasm, etc).
In other words, identical to a Masson Trichrome, but with yellow RBCs
instead of red. Very good method for seeing the RBCs (yellow) inside the
fibrin mesh (red). As compared with PTAH, where everything is blue except
the collagen. Or Masson trichrome where RBCs and fibrin are both red.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Tuesday, October 31, 2006 11:36 AM
To: Henry, Charlene; Histonet@lists.utsouthwestern.edu
Subject: Fibrin stain Re: [Histonet] Serum Fiber in Blood

Martius Yellow also known as MSB stain for fibrin works on FFPE.  I will
attach the method to you privately.  You may also find this on a staining
website.  Peggy Wenk kindly sent this method to me so a thank you goes out
to her.

At 08:51 AM 10/31/2006, you wrote:
>Our mouse pathologist has previously ordered the PTAH stain for blood 
>serum fiber in mouse tissues; however we plan to retire this stain 
>because of the mercury used in the stain. This is the last place in the 
>lab that requires mercury. My question is: do any of you know of 
>another special stain or antibody that will detect blood serum fiber in 
>FFPE mouse tissue?
>Thanks,
>Charlene
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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