[Histonet] virbratome sections and staining

From:William Ares

I have recently started sectioning by vibratome and had some questions regarding staining, particularly H&E (hematoxylin and eosin).  After sectioning, I am plating the sections on glass slides and allowing the buffer to evaporate.  When reinserting the slides into buffer for H&E staining the sections fall off.  If anyone else has done this, what methods do you use to make sure you do not lose your sections.  Thanks.

William Ares
Labratory of Molecular and Developmental Neuroscience
Weill Medical College of Cornell University

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