[Histonet] Timms stain in fresh frozen cryosections
From: | "Anna Elisse Beaudin" |
Hello,
I would like to do Timm's stain in fresh frozen cryosections of mouse
brain. The only protocol I could find involves incubating the sections
O/N in a dessication chamber with highly toxic H2S gas (created by
adjusting a 0.1% Na2S solution to pH 7.3) prior to rehydration followed
by classic timms staining protocol. I was wondering if anybody had any
other protocols and/or the best way to do this without poisoning myself
and my lab mates :) I really appreciate any help!
Thanks in advance,
Anna Beaudin
Division of Nutritional Sciences
Cornell University
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