[Histonet] Questions about muscle FIBER TYPE differentiation
I'm a new member in histonet. I've been doing some IHC and IF staining
on frozen muscle sections from mice.
I've encountered a metachromatic dye-ATPase staining method which was
originally reported by Ogilvie RW on Stain Technology (1990) Vol 65,
No.5, 231-240 and subsequently used by various labs to identify muscle
fiber types. In short, they claimed that using this method, all fiber
types could be identified simultaneously by different intensity and
homogeneity of staining.
I've followed the protocol three times so far. It did not show as pretty
as others. I'm wondering if anyone has tried this method and what steps
or modifications need to be done to optimize it. Also how is the result
compared to IHC or IF using different anti-myosin antibodies?
University of Texas
Health Science Center
at San Antonio
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