Hi histoneters!  I was looking for the recipe to make Tris EDTA pH 9 for antigen retrieval.Everybody mentions using EDTA, but judging by the molarity of the solution and the quantity to add I guessed that in fact it is not EDTA (M = 292,24), but a di-sodium, di-hydrated salt (M =372,24) also known as titriplex III.Is this correct?Does anybody know if the Na is essential for the retrieval action of thebuffer?If I do not have Titriplex III and I just use EDTA, would it be enough?Many thanks from now!Daniel Pirici, MDUniversity of Medicine and Pharmacy, Craiova, Romania

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