[Histonet] EDTA Decal endpoint


Hi all,

I am about to start decalcifying rat femur and sternum for myelofibrosis
evaulation through the use of EDTA. (unless anyone has another maybe faster
methhod that will not destroy reticulin or interfere is Gomori Silver
stain).  I wanted to know if there was a definitive endpoint, rather than
"when the bone is floppy" etc.  I spoke with a colleague who said there may
be a commercially available testing method, by color change.  Is this true,
and if it it does exist, what is it called?

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