[Histonet] Artifacts- condensate like

From:"Botsford, Daniel"


Is there a remedy for condensate artifact? There is complete lost of nuclear
detail in the cell. The nucleus stains homogenous light blue or white. This
artifact was generated in prostate biopsies after a machine malfunction.
Tissue processer  reagents leaked into the waxes.

I have seen the condensate artifact in GI biopsies before if the alcohol are
not changed frequently and the absolute alcohol becomes contaminated with
water and carrys over to the xylol. I have heard of it occurring on weekend
runs when the formalin condenses on the lid of the retort and drops back
into nonaqueous solutions during processing. I have never heard of a remedy.


Daniel Botsford
Windsor Regional Hospital
1995 Lens Avenue
Windsor, Ontario
N8W 1L9
519-254-5577 ext 52373
519-254-6861 fax

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