Re: [Histonet] general mouse-ihc-stain
Anti-mouse IgG stains all IgG in mouse tissue which is located in the
blood which fully perfuses organs and tissues from the mouse. The IgG
may also be within the extracellular matrix outside the vasculature.
It does not stain ALL cells at all but rather specifically stains the
IgG in the blood and that which has diffused into the tissues. There
is something on the order of 10mg IgG per ml blood so you can imagine
why it gives an intense staining pattern ... that is 1000X the amount
of primary we normally add for staining!!!! To address your final
question more specifically, anti-mouse IgG is NOT a marker of mouse
cells in general as if you stain mouse cells in vitro (therefore
without the blood perfusion) you will get NO STAINING.
At 11:33 AM +0200 10/8/06, Gudrun Lang wrote:
>perhaps not a really clever question, but I hope somebody can easily explain
>it to me. Why does a (monoclonal) anti-mouse antibody stain all
>mouse-tissue, when it is created against the fc-part of mouse-antibodies? Or
>does this only happen with polyclonal antibodies, that are nt really
>purified? Is such a secondary anti-mouse ab specific against mouse-tissue in
>general? Is this just an artefact, that could be used for identifing
>mouse-cells in other species? Hmmm.
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