Re: [Histonet] counterstain for immunofluoroscence
If you wanted a flourescent nuclear counterstain you could use DAPI or
Hoechst. There are mounting mediums that already have DAPI in them like
SlowFade Gold with DAPI.
Tom Pier
>>> "Anna Elisse Beaudin" 10/11/06 7:38 AM >>>
Hello,
I am processing mouse brain sections for double-immunofluorescence
and
need to find a suitable counterstain. I do not necessarily need the
counterstain to be fluorescent, since the purpose of the counterstain
is only to map the area of interest, but I want to make sure that
whatever counterstain I use is not going to quench my fluoresence. I am
using secondary-conjugated Alexa 488 and Streptavidin-Alexa 546. I
appreciate any and all advice. Thanks in advance for your help!
Best,
Anna Beaudin
Division of Nutritional Sciences
Cornell University
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