Re: [Histonet] counterstain for immunofluoroscence

From:"Thomas Pier"

If you wanted a flourescent nuclear counterstain you could use DAPI or
Hoechst.  There are mounting mediums that already have DAPI in them like
SlowFade Gold with DAPI.

Tom Pier

>>> "Anna Elisse Beaudin"  10/11/06 7:38 AM >>>

   I am processing mouse brain sections for double-immunofluorescence
need to find a suitable counterstain.  I do not necessarily need the
counterstain to be fluorescent, since the purpose of the counterstain
is only to map the area of interest, but I want to make sure that
whatever counterstain I use is not going to quench my fluoresence. I am
using secondary-conjugated Alexa 488 and Streptavidin-Alexa 546.  I
appreciate any and all advice.  Thanks in advance for your help!

Anna Beaudin
Division of Nutritional Sciences
Cornell University

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