Re: [Histonet] buffer for mounting floating brain sections

From:Geoff McAuliffe

Hi Caroline:

    PBS IS phosphate buffer with saline. I have had the same problem you 
are experiencing and I used a dilute phos. buffer. 0.05 M without saline 
to mount the sections. As soon as the sections are adhered I rinsed 
gently with distilled water and blot off the excess. Time consuming but 
it worked.


Caroline Bass wrote:

> Hey guys,
> I know this is a question that has probably popped up before, but I  
> searched the archives and didn't find exactly what I need.
> I want to mount floating mouse brain sections (40 micron, NBF fixed,  
> sucrose protected) onto superfrost slides.  I will immediately  
> coverslip after drying, they will not be processed further.  What is  
> the best buffer to float the sections in when mounting onto the  
> slides.  PBS seems to leave a lot of crystals, I have heard that 0.1M  
> phosphate buffer is better.  Distilled water results in a lot of  
> curls.  Since I am not going to process the slides I don't have to  
> worry about them falling off later (which I heard is a problem with  
> charged slides and PBS).
> If there is a preferred buffer could you also include the recipe.
> Thanks,
> Caroline
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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