Re: [Histonet] Why ammonia water?

From:Rene J Buesa

  Ammonia water is an alkaline solution and, as all alcaline solutions (soap included), it has softening qualities (as when you feel your hands softer after washing your hands with soap).
  Since the solution can also "penetrate" and "soak" the processed tissue, it makes it softer and easier to section, specially decals.
  René J.

Paula Sicurello  wrote:
Hello Histonetters,

The question of the day, around here = anyway, What is the history of
using 3% ammonia water while sectioning para= ffin?

We use= it for small biopsies and skins but we don't know why and who
established = that protocol anyway.

So if anybody out there knows why, please let me know.

Paula :-)

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