I have been using a rabbit polyclonal from Abcam on (ab616) @ 1:800, HIER overnight at 60-65C in Biocare Reveal or BORG (don't use the BOG on tissues that are really fatty, like skin or brains - they will fall off!).  The BORG gives better staining, but the Reveal is acceptable if using fatty tissues. Kim MerriamCambridge, MA----- Original Message ----=0AFrom: Jeanne Estabel To: histonet@lists.utsouthwestern.eduSent: Wednesday, October 11, 2006 5:09:32 AMSubject: [Histonet] Monoclonal antibody to Beta-galactosidaseHi, I need to find a monoclonal antibody to Beta-galactosidase working onFFPE mouse tissue.Could you give me some advice?ThanksJeanne Estabel, PhDMGP Histologist, Team 109Wellcome Trust/Sanger InstituteHinxtonCB10 1SAUK_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet=0A
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