Re: [Histonet] BSL precautions for Primate frozen sectioning?

From:"Andrea T. Hooper"

Even so, wouldn't you expect to treat it just 
like human tissue which could potentially also be 
hepatitis, HIV infected ... aka with universal 
precautions? Aren't we supposed to consider all 
human tissue as having those diseases and worse 
even if patient history is known, per universal 
precautions? The major thing to consider I 
believe is that primates commonly are infected 
with a potentially deadly form of hepatitis if 
humans get infected. Maybe some of you guys from 
SNBL or the like can speak up?

>   I strongly recommend you to try to find out 
>EXACTLY the conditions of those baboons.
>   Remember that human AIDS virus is a mutant 
>from a chimpanzee AIDS like virus!
>   You should not mess around with unknown things after all!
>   René J.
>Kim Kusser  wrote:
>   Hi,
>I need a little (or, more than likely, a lot) of advice. I do a lot of
>frozen sectioning on mouse tissues. Our facility is only mouse. A
>researcher wants to be able to section baboon (uninfected and
>unfixed...just OCT embedded) tissue. Our cryostats are BSL2 (lab coats
>and gloves).
>To the people out there in the know, how do you treat Primate tissue
>when it comes to frozen sectioning in Cryostats? Would this procedure be
>recommended to all lab personnel without knowledge of their immune
>status (i.e. immunocompromised staff)?
>Thanks you for any and all help.
>Kim Kusser


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