RE: [Histonet] tissue coming off slides
We use regular slides to pick up our frozen sections when we are doing
routine H&E's. We fix the slide in Rapid-Fixx(Thermo Electron Corp.) prior
to staining and the sections never fall off. Good Luck!
Christie Gowan
>From: John Baker
>To: Histonet
>Subject: [Histonet] tissue coming off slides
>Date: Thu, 5 Oct 2006 12:00:46 -0400
>
>Hi All, A group I work with just came to ask if there was a way of keeping
>frozen tissue sections from coming off a slide? It seems there technician
>used uncoated/regular slides to mount 100's of sections. The tissues were
>embedded in OCT and cut at 5 microns but of course are sliding off during
>any staining procedure. Is there a protocol of any sort to coat the slides
>after the fact to retain the sections through staining? I at least
>suggested they stain flat. Any suggestions are appreciated. John
>
>John A. Baker
>The University of Michigan
>Orthopaedic Research Laboratories
>Histology Unit
>109 Zina Pitcher Place, 2218 BSRB
>Ann Arbor, MI 48109-2200
>734-936-1635
>
>
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