RE: [Histonet] tissue coming off slides

From:"Weems, Joyce"

Might try putting a bit of 37% formaldehyde in the bottom of a coplin and see if the vapor would fix the section enough to hold it on the slide. Good luck!

-----Original Message-----
[]On Behalf Of John
Sent: Thursday, October 05, 2006 12:01 PM
To: Histonet
Subject: [Histonet] tissue coming off slides

Hi All,  A group I work with just came to ask if there was a way of 
keeping frozen tissue sections from coming off a slide?  It seems there 
technician used uncoated/regular slides to mount 100's of sections.  
The tissues were embedded in OCT and cut at 5 microns but of course are 
sliding off during any staining procedure.  Is there a protocol of any 
sort to coat the slides after the fact to retain the sections through 
staining?  I at least suggested they stain flat.  Any suggestions are 
appreciated.  John

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200

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