RE: [Histonet] tissue coming off slides

From:"Zajic Kari"

Hi John!
That used to happen to me and I changed all of my slides to + charged slides and it stopped. Now, I put the sectioned slides directly into Hematoxylin for staining, but I have had techs feel more comfortable putting them in 95% Alcohol first to fix. I personally haven't had negative or positive feedback on the stain quality either way (my Pathologists didn't notice a difference). 
I also heard just yesterday from a tech that she puts them into Formalin before staining...this I have never heard before, but she claims it works. 
Good luck, hope this helped a tad...
Kari :)  

Kari Marie Zajic HTL,MLT
Histology/Pathology Supervisor
Palms West Hospital
Pathology Department
13001 State Road Eighty
Loxahatchee, Florida 33470
phone:      (561)798-6036
fax:             (561)753-4298
voicemail: (561)753-4299
pager:        (561)610-4949

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-----Original Message-----
[]On Behalf Of John
Sent: Thursday, October 05, 2006 12:01 PM
To: Histonet
Subject: [Histonet] tissue coming off slides

Hi All,  A group I work with just came to ask if there was a way of 
keeping frozen tissue sections from coming off a slide?  It seems there 
technician used uncoated/regular slides to mount 100's of sections.  
The tissues were embedded in OCT and cut at 5 microns but of course are 
sliding off during any staining procedure.  Is there a protocol of any 
sort to coat the slides after the fact to retain the sections through 
staining?  I at least suggested they stain flat.  Any suggestions are 
appreciated.  John

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200

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