RE: [Histonet] tissue coming off slides
That used to happen to me and I changed all of my slides to + charged slides and it stopped. Now, I put the sectioned slides directly into Hematoxylin for staining, but I have had techs feel more comfortable putting them in 95% Alcohol first to fix. I personally haven't had negative or positive feedback on the stain quality either way (my Pathologists didn't notice a difference).
I also heard just yesterday from a tech that she puts them into Formalin before staining...this I have never heard before, but she claims it works.
Good luck, hope this helped a tad...
Kari Marie Zajic HTL,MLT
Palms West Hospital
13001 State Road Eighty
Loxahatchee, Florida 33470
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[mailto:firstname.lastname@example.org]On Behalf Of John
Sent: Thursday, October 05, 2006 12:01 PM
Subject: [Histonet] tissue coming off slides
Hi All, A group I work with just came to ask if there was a way of
keeping frozen tissue sections from coming off a slide? It seems there
technician used uncoated/regular slides to mount 100's of sections.
The tissues were embedded in OCT and cut at 5 microns but of course are
sliding off during any staining procedure. Is there a protocol of any
sort to coat the slides after the fact to retain the sections through
staining? I at least suggested they stain flat. Any suggestions are
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
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