RE: [Histonet] The procedure that substitues xylene with mineral oil.

From:Rene J Buesa

Gayle:
  It is evident that you have not read the procedure!
  Used minaral oil (= paraffin oil = paraffin of low molecular weight) is MIXED with the used paraffin and together form a solid mass to be incinerated after cooling.
  By the way, it is René (male, just one "e")
  René J.

Gayle Callis  wrote:
  Phil and Renee,

If the solvents i.e. alcohols, xylene or xylene substitutes need to be 
collected for incineration after CJD cases, wouldn't mineral oil also need 
to be collected and disposed of in the same way? The expense of collection 
will still be there no matter what the powers that be do not like to 
do. Mineral oil may be less toxic compared to xylene but after 
contact.contamination with CJD or any other other prion diseased 
tissues, it's status changes back to being a collectable solvent.

We work with prion diseased animal tissues using xylene substitutes (they 
work very well for processing and staining) and we are REQUIRED to collect 
these for incineration.

At 11:23 AM 10/1/2006, you wrote:
>I am attaching the procedure under separate cover.
>René J.
>PhiHo Hoang 
wrote:
>Greetings,
>I am a new subscriber to this list and I am reading the list archive.
>I came across a posting from René J. responding to a request from Sharon:
>Sharon:
>I am sending privately a procedure I have that substitutes xylene with 
>mineral oil. It is 100 times less toxic than xylene and 3 times cheaper.
>René J.
>Sharon Allen <@t>exchange.hsc.mb.ca> wrote:
>Does anyone use xylol substitutes, if so how good are they?
>We do CJD testing & "the powers that be" don't like to pay for getting rid
>of contaminated xylol. I don't want to have to start testing different
>substitutes, so any help would be greatly appreciated.
>Thanks
>Sharon
>sallen <@t> hsc.mb.ca
>I am interested in this procedure
>It is very much appreciated if I can get a copy
>of that procedure.
>Best regards,
>PhiHo
>
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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