RE: [Histonet] Re: Superfrost slides-Histonet question

From:"Liz Chlipala"

I have to agree with Gayle I have Pathology Innovations chucks, base molds,
etc.  They work great, much better than the ones you get when you buy your
cryostat.  I frequently get frozen tissue that is not in OCT and the base
molds from PI work great, I have embedded up to 5 different mouse tissues in
the larger one and do not have any problems with sectioning.  They also work
great for fixed frozen sections. It's a great tool.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Gayle Callis
Sent: Tuesday, October 03, 2006 2:11 PM
To: Stephen Peters M.D.;
Subject: Re: [Histonet] Re: Superfrost slides-Histonet question

Hi Stephen,

You are very welcome!  It was a pleasure to visit with you at Phoenix NSH 
S/C.  Keep up the good work creating those clever tools you have for 
cryosectioning!!!  Your waffle weave "grid" (square and rectangular) metal 
disks are superbly designed for holding frozen tissue blocks - the BEST!!! 
especially for undecalcified bone cryosectioning and they fit in my 1850's, 
all three of them!

Take care

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717

  At 12:01 PM 10/3/2006, you wrote:
>Hi Ion,
>   In our Leica !850's I use a blade angle of 5 with our low profile 
> blades. I know I
>   would not have much luck at a negative angle.
>    As far as curling tissue, in my experience the best temperature for 
> cutting
>    most tissus (except fat) is the warmest temp that the tissue will cut
>   without crumpling. Try starting  to cut a touch on the warm side. Press 
> a piece
>   of cryostat temp metal ( I use my over-chuck blocks for this) on the 
> block face a
>    few seconds at a time until the tissue starts to cut. When the tissue 
> is just
>   cold enough to cut it will float off in a flat sheet. This is why the 
> sections in my
>   videos lie so flat. It is largely do to the correct temperature. As we 
> cut colder and colder we get more and more curling and shattering. If you 
> are starting with a very
>   cold block  warming with the thumb will get you down to the same temp. 
> But if it
>    takes more than a few seconds to get your section, warming will need 
> to be
>   repeated as the temp will quickly fall back down to the core temp of 
> the block.
>    Thanks for visiting my web site.  Gail thanks for the complement it 
> means a lot coming from you.
>Stephen Peters M.D.
>Vice Chairman of Pathology
>Hackensack University Medical Center
>201 996 4836
>Pathology Innovations, LLC
>410 Old Mill Lane,
>Wyckoff, NJ 07481
>201 847 7600
>Histonet mailing list

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