RE: [Histonet] Collagen immunohistochemistry in sheep

From:"Liz Chlipala"


I have not ran collagen immunostains specifically on sheep, but we have run
them on mouse, rat, human and goat bone sections that have been formic acid
decaled and the antibodies that are listed below work for those species so I
suspect that they will also work for sheep.

Collagen I - Rabbit Polyclonal - Biogenesis
Collagen II - Rabbit Polyclonal - MD biosciences
Collagen III - Rabbit Polyclonal - Biogenesis

Hope this helps.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of
Sent: Wednesday, October 11, 2006 2:14 AM
Subject: [Histonet] Collagen immunohistochemistry in sheep

Hi Histonetters,
does anyone have experience with immunos to differentiate collagen I vs. III
(vs. VI) in sheep 
tissue ? The tissues are already formalin fixed and decalcified with EDTA.
Any suggestions which antibodies are suitable and which protocols are best
are welcome !

Thanks a lot in advance
Best regards

PD Dr. med. Reinhard von Wasielewski

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