[Histonet] how to count nodules in a xenograft model

From:"Caroline Bass"

Hello Everyone,

I am posting this on behalf of a friend.  I know very little about  
xenograft models and I thought someone on this list may have more  

Essentially, she is injecting MDA-MB-231 cells i.v. in mouse and  
studying metastasis.  She wants to get a count of the nodules that  
form in the lung.  Does anyone have a good protocol for doing this.   
She has little histology skills, so most likely she will send the  
tissue to a lab to process.  What is the safest way to collect the  
tissue, how should it be processed, and how does one get a good idea  
of the number of nodules formed?

Any help would be appreciated.



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