[Histonet] embedding/freezing samples from glycerol
|From:||"Anna Elisse Beaudin" |
I recently froze embryo samples in OCT that had been sitting in 70%
glycerol. When I tried to section these samples on the cryostat, the
results were terrible -- the specimen basically would not be cut
(appeared gooey and stuck out of the block). I wasn't able to save
anything. The problem I have is that all of my samples are in glycerol,
and I would eventually like to section them. :) Does anyone have any
advice as to what I can do with these samples to make them section-able?
They have been stained with Xgal, so I can't do anything that would
weaken the stain. Thanks in advance for any advice!
Division of Nutritional Sciences
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