[Histonet] embedding/freezing samples from glycerol

From:"Anna Elisse Beaudin"


  I recently froze embryo samples in OCT that had been sitting in 70%
glycerol.   When I tried to section these samples on the cryostat, the
results were terrible -- the specimen basically would not be cut
(appeared gooey and stuck out of the block).  I wasn't able to save
anything.  The problem I have is that all of my samples are in glycerol,
and I would eventually like to section them. :)   Does anyone have any
advice as to what I can do with these samples to make them section-able?
 They have been stained with Xgal, so I can't do anything that would
weaken the stain.  Thanks in advance for any advice!

Anna Beaudin
Division of Nutritional Sciences
Cornell University

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