[Histonet] buffer for mounting floating brain sections
I know this is a question that has probably popped up before, but I
searched the archives and didn't find exactly what I need.
I want to mount floating mouse brain sections (40 micron, NBF fixed,
sucrose protected) onto superfrost slides. I will immediately
coverslip after drying, they will not be processed further. What is
the best buffer to float the sections in when mounting onto the
slides. PBS seems to leave a lot of crystals, I have heard that 0.1M
phosphate buffer is better. Distilled water results in a lot of
curls. Since I am not going to process the slides I don't have to
worry about them falling off later (which I heard is a problem with
charged slides and PBS).
If there is a preferred buffer could you also include the recipe.
Histonet mailing list
<< Previous Message | Next Message >>