[Histonet] Using lectin BS-1 for staining endothelial cells
Hello Histonetteers,
I am having problems with the staining of rat endothelial cells in
paraffin sections using a biotin labeled lectin from Bandeiraea
Simplicifolia BS-1 (from Sigma). The lectin does not appear to be
staining any of the endothelial cells. I have tried using citrate buffer
for antigen retrieval and DAB, DAB + NiCl or FICT to visualize the site
of reaction. Sections are from ovary grafts and have very new blood
vessels forming. Any information would be helpful.
Thanks
Gabriel Anesetti
Histology and Embryology Departament
School of Medicine
Montevideo, Uruguay
ganesett@fmed.edu.uy
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