[Histonet] Using lectin BS-1 for staining endothelial cells

From:Gabriel Anesetti

Hello Histonetteers,
I am having problems with the staining of rat endothelial cells in 
paraffin sections using a biotin labeled lectin from Bandeiraea 
Simplicifolia BS-1 (from Sigma). The lectin does not appear to be 
staining any of the endothelial cells. I have tried using citrate buffer 
for antigen retrieval and DAB, DAB + NiCl or FICT to visualize the site 
of reaction. Sections are from ovary grafts and have very new blood 
vessels forming. Any information would be helpful.
Gabriel Anesetti
Histology and Embryology Departament
School of Medicine
Montevideo, Uruguay   

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