[Histonet] Re: Superfrost slides-Histonet question
|From:||"Stephen Peters M.D." |
In our Leica !850's I use a blade angle of 5 with our low profile blades. I know I
would not have much luck at a negative angle.
As far as curling tissue, in my experience the best temperature for cutting
most tissus (except fat) is the warmest temp that the tissue will cut
without crumpling. Try starting to cut a touch on the warm side. Press a piece
of cryostat temp metal ( I use my over-chuck blocks for this) on the block face a
few seconds at a time until the tissue starts to cut. When the tissue is just
cold enough to cut it will float off in a flat sheet. This is why the sections in my
videos lie so flat. It is largely do to the correct temperature. As we cut colder and colder we get more and more curling and shattering. If you are starting with a very
cold block warming with the thumb will get you down to the same temp. But if it
takes more than a few seconds to get your section, warming will need to be
repeated as the temp will quickly fall back down to the core temp of the block.
Thanks for visiting my web site. Gail thanks for the complement it means a lot coming from you.
Stephen Peters M.D.
Vice Chairman of Pathology
Hackensack University Medical Center
201 996 4836
Pathology Innovations, LLC
410 Old Mill Lane,
Wyckoff, NJ 07481
201 847 7600
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