[Histonet] RE: Histonet Digest, Vol 35, Issue 21

From:"Kewley, Sharyl F."

Hi Jason,
I have checked in Conn's Biological Stains and for the Luxol Fast Blue dye it states that the C.I. is Solvent Blue 38, so I believe that is what you will want to use.  You stated that Sigma made a 37 and a 38, you will need to order Solvent Blue 38 from Sigma. 
I am not sure what the difference is between Solvent Blue 37 and 38, but, in the Sigma catalog if you will notice the 37 says only practical grade and the 38 says Luxol Fast Blue MBSN and then practical grade.  So to me that means that only the 38 pertains to the Luxol Fast Blue staining.
If you have access to a Conn's Biological Stains book it will give you lots of information on all different staining dyes.  
Hope this helps,
Sharyl Kewley, HT (ASCP)
Columbia Regional Hospital
404 Keene St.
Columbia, MO  65201



Message: 25
Date: Tue, 10 Oct 2006 22:35:07 -0300
From: "Jason Potas" 
Subject: [Histonet] help with luxol fast blue myline staining
To: Histonet 
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi everyone,

im trying to make up Luxol Fast Blue to look at myelin in frozen cut spinal
cord sections.

Sigma makes Solvent Blue 37 and Solvent Blue 38. Which is better or
the correct one to use?

does anyone know what the difference is between the two?

many thanks


Jason Potas (PhD)
Laboratorio de Neurobiologia Celular e Molecular
Instituto de Biofisica Carlos Chagas Filho - IBCCF
Universidade Federal do Rio de Janeiro
CCS bloco J sala J1-029 Ilha do Fundao
21941-590 Rio de Janeiro, RJ - Brasil
Tel: +5521 2280 4694
      +5521 2562 6554
Email: jason@biof.ufrj.br



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