[Histonet] Proof that fixation in formaldehyde solution can be reversible?
I am in a heated debate with an investigator regarding his samples. They were shipped to me after (24-48hrs fixation - 10% NBF or 4% PF unsure exactly which one) and then switch to PBS for shipment. They sat at room temperature for over 1 month before being processed routinely and embedded in paraffin wax.
Can someone tell me if indeed the fixation is reversible, or once they have been fixed for over 24hrs they remain fixed?? Can anyone refer me to books or papers that talk about this??
Also, what affect on antigenicity will such storage in PBS have on these samples?
Thank you all so much for your help.
NBF, Alcohol, 10%, paraformaldehyde, 4%, fixation, fix, ethanol, 70%, reverse, reversible.
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