I used the DAKO CD117 (rabbit Ig) at 1:25 with HIER at pH 6.0 in the
steamer (20 min in + 20 min out) without any problem (in human tissue with
human melanoma control).
Are you running a "other than rat" parallel control to be sure that your
protocol is working OK?
Rene J.

b003046@nf.au.dk wrote:
Hi, im trying to get the DAKO polyclonal rabbit anti-human c-kit (CD117) to
work
in rats in paraffin. I have tryed using antigen retrieval in microwave and down
to 1:50 dilution. Also I have tryed the C-19 c-kit from Santa Cruz and used
both on paraffin and frozen tissue. It just doesnt want to work! Can anybody
help?

Thanks,
Mette K. Hagensen
Department of Cardiology B, Research Unit
Aarhus University Hospital, Skejby Hospital
Brendstrupgaardsvej 100
8200 Aarhus N
Denmark

Phone: +45 89496237
Fax: +45 89496009
Mail: b003046@nf.au.dk


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