Re: [Histonet] decalcifying solutions for skeletochronology

From:John Kiernan (by way of histonet)

Dear Dr McCallum,

Every book in the field of histotechnology contains
several recipes for decalcifying. The calcified material
can be removed by dissolving in a suitable acid (usually
hydrochloric or formic) or by chelation with a suitable
anion (usually citrate or EDTA). The choice of method is
based on the requirements of the investigation.

If you are in doubt about choosing a method, ask the
internet for references rather than recipes.
There are scores of books and hundreds of papers!
A solid place to start searxhing the literature is
Pearse's Histochemistry.

John A. Kiernan MB, ChB, PhD, DSc
Professor, Dept of Anatomy & Cell Biology
The University of Western Ontario
London, Canada.
Malcolm McCallum wrote:
> Hi,
> I am having some students do various skeletochronology projects with
>amphibians and reptiles.  I could just buy some decalcifying solution, but
>we have so many acids, I figured I would make some.  Any good recipes out
>there for decalcifying bone w/o heating?
> Malcolm L. McCallum
> Assistant Professor
> Department of Biological Sciences
> Texas A&M University Texarkana
> 2600 Robison Rd.
> Texarkana, TX 75501
> O: 1-903-233-3134
> H: 1-903-791-3843
> Homepage:

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