Re: [Histonet] alcian blue and alizarin red

From:Rene J Buesa (by way of histonet)

Alizarin red was the standard procedure I used for calcium (carbonate /
phosphates) pretty much as descibed by Sheehan & Hrapchak [(1973):153-4]
The solution has to have pH adjusted to 4.1-4.3 and final dehydrating has
to be done with acetone (as ethanol will remove the stain).
Alcian blue is standard for mucosubstances [Luna,1968:163] but I never used
both combined.
Both procedures on sections of paraffin embedded tisusues.
Rene J

"Walters, Katherine S"  wrote:
Has anyone done alcian blue/alizarin red staining on tissue sections?
The protocols I've turned up seem to be for cleared tissue.


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