Re: [Histonet] Re: Tissue with Fat

From:"Instrumedics" (by way of histonet)

The CryoJaneTape-Transfer system makes it possible to cut fat with
remarkable success. The tape adhered to the block face captures the section
as you cut the section. You then transfer this intact section to an adhesive
coated slide.

Please see the details on our web site or contact us
with your questions.

We have a CD-R that we can send you with a demonstration of the entire
CryoJane process from snap-freezing the tissue on the Gentle Jane to cutting
the section on to the tape and finally transferring it to the slide.


----- Original Message -----
From: "Arshia Mian" 
Sent: Thursday, October 13, 2005 12:32 PM
Subject: [Histonet] Re: Tissue with Fat

I have been having some problems with cutting Tissue with Fat on a
cryostat (box temp and

object temp are at -20C).  The slices I cut tend to curl in the middle
of the block close to the tissue. I have

changed the blade several times, but it still continues to happen even
with a new blade. Is there any correlation

between the box temp and the object temp that would cause this to
happen? Any suggestions?

I have also noticed that at times, even though my setting is set to 20
microns, that I get thick and thin cuts.

Any suggestions on this as well? I would greatly appreciate it. Thanks.

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