Re: [Histonet] Perfusion/Dye

From:Rocan (by way of histonet)

Hi Katie,
You need to incorporate something that does not diffuse outside the
vascular walls or gets washed away with the perfusion.  We use
lectins such as  LEA (Licopersicum esculentum agglutinin (Tomato
lectin).  You must inject before you start the perfusion and allow it
to circulate.  Dr. Donald McDonald at UCSF has many papers published
where they inject such lectin.  You may want to see his papers on
PubMed.  We use about a tenth of the doses he uses and works just
fine.  All the vessels will be labeled.  You can buy LEA labeled with
fluorescent molecules, with biotin, HRP, etc.  It is not very
expensive and the labeling is gorgeous. You can contact me directly
if you have any questions.  I hope this helps
Good luck,

Dr.Rocio Sierra-Honigmann
Engineered Wound Repair Laboratory
Cedars Sinai Research Institute
Davis 1091

On Oct 14, 2005, at 4:41 AM, Crosby, Katherine wrote:

> Hi All,
> I am looking for suggestions of dyes, fluorescent or otherwise,
> that could be incorporated into a standard mouse perfusion procedure.
> Thanks in advance,
> Katie
> Katie Crosby
> Manager, Immunohistochemistry
> Cell Signaling Technology
> 166B Cummings Center
> Beverly, MA  01915
> Phone: 978-867-2352
> Fax:  978-867-2402
> E-mail:
> _______________________________________________
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