Re: [Histonet] Mechanical tool for removing spinal cord from rats.
|From:||"John A. Kiernan" (by way of histonet)|
A simple procedure is as follows:
1. Remove the head.
2. Cut through the spinal column just above
3. Insert the nozzle of a 2 ml syringe
(filled with saline) into the spinal canal
and push the plunger.
4. Catch the extruded cord (and cauda equina)
in a small beaker of saline.
The upper nerve roots are avulsed by this
method but the histology of the cord itself
is well preserved. Biochemists have used this
technique for many years. It was published as
"new" from an anatomical perspective
24 years ago.
MEIKLE ADS & MARTIN AH 1981
A RAPID METHOD FOR REMOVAL OF THE SPINAL-CORD
STAIN TECHNOLOGY 56 (4): 235-237.
(According to Web of Science this paper has
been cited 16 times.)
Alison Meikle was an MSc student in our
department, supervised by Sandy Martin.
If cou need attached spinal nerves rostral
to the cauda equina this is not the method
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
> What do people use to remove spinal cords from rats during post mortems??
> At present we use large scissors to cut through the bone but are looking to
> try a mechanical tool to replace this.
> Does any one have any recomendations.??
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