Re: [Histonet] Immunohistochemistry questions

From:Andrea Hooper (by way of histonet)

If you look at your sections with no primary and no secondary you should be
able to determine if it is
autofluorescence or not. It is my experience that paraffin embedded bone is
highly autofluorescent and needs to
be blocked prior to staining.

If you provide more details of your protocol perhaps we can help.

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