Re: [Histonet] Immunofluorescence - CAP ?

From:Patti Loykasek

Laura, for IgG, IgA, and IgM we use normal tonsil that has been frozen. For
the others we use past positive cases. Hope this info helps.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA


> A new CAP (College of American Pathologists) question has appeared on our
> checklist for the next inspection. The question asks, "Are appropriate
> positive and negative controls performed with each case tested using
> immunofluorescence?"  Sometimes positive controls not abundant and since we
> are working with only frozen tissue, does anyone have any helpful ideas? Other
> than past positive cases, is there any other positive tissue that we could use
> for IgA, IgM, IgG, C3, Fibrinogen, properdin and/or C4?
> Thanks much,
> Laura 
> Laura Bliven
> Histology Lab/IHC
> Marshfield Laboratories
> 1000 N. Oak Ave.
> Marshfield, WI 54449
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