Even -80 is not cold enough, but the colder the better.  The ice will
gradually restructure as crystalline ice, and expand.  This will break
cell membranes and disrupt the tissue quality. Use as early as possible.
See the link:
http://www.myneurolab.com/global/Manuals/Tips%20and%20Techniques%20Freez
ing%20Artifact.pdf


Cordially,
Charles W.  Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300 x 342
FAX  314 522 0377
cwscouten@myneurolab.com
http://www.myneurolab.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily
Jane Wiesner-Camm
Sent: Tuesday, October 18, 2005 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Storing frozen rat brain slices

Question: I was wondering if anyone could let me know what is the
optimal storage for frozen rat brain slices (approx 2mm thick) which are
in OCT: -20 degrees celsius or -80?
Thanks in advance,
Emily


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