From:"Favara, Cynthia (NIH/NIAID)" (by way of histonet)

I have this paper and hope to try this next week. Note that the fixative is
picric acid/paraformaldehyde/gluteraldehyde. I would love to get great
fixation with 1ml and without opening the chest cavity. I will let you know!


Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

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-----Original Message-----
From: Adam Perry []
Sent: Thursday, October 13, 2005 8:20 AM
Subject: [Histonet] MOUSE BRAIN FIXATION

I downloaded the technical report on this variant perfusion technique.  I'm
amazed that you can get good fixation of the brain with 1 ml of fixative
pumped through the heart without clamping off descending aorta, etc.  Has
anyone tried this technique (or similar variants), and what kind of results
were obtained?

I'm planning on trying it in a week or so, because I would love to get good
brain fixation with so little time, fixative and waste production...but am

Adam Perry

Department of Physiology and Biophysics
University of Illinois
Chicago, IL 60612

There have been several inquiries regarding fixation of mouse brains

A short technical report has just been published in BioTechniques:

Minimally invasive method for murine brain fixation
Eichenbaum KD et al
Biotechniques 2005; 39(4): 487-500

After simple registration, it can be accessed at 

Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax

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