RE: [Histonet] How do you get red pulp really red?

From:"Anne Van Binsbergen"

Hi Mel
Eosin in 70% alc....
 - what % eosin
- is it eosin Y or G
I use a working solution of roughly 0,5% eosin Y in dist H2O to which I
add a few drops of acetic acid and a 'dash' of 1% aq phloxine 
For frozens we use a 1% with a few drops of acetic acid - faster
Its really all a matter of timing, which also depends on staining method
(manual, auto, etc) - trial and error should get you there
Remember that it isn't always the one stain/colour (Haem or Eos) which
is the culprit - it's the balance between them
If your water/section is too alkaline the eosin will not stain the
section - so wash really well after 'blueing'
When I took over this lab they were using alc eosin and the Paths hated
the staining
Now they LOVE it.

-----Original Message-----
From: [] 
Sent: Tuesday, October 25, 2005 5:04 AM
Subject: [Histonet] How do you get red pulp really red?

Hello everyone,
I teach a histology class and often have the students make their own 
I notice that my very old histology slides have very bright red pulp and

dark white pulp. I assume they were prepared using H&E staining. I have 
tried but the red is never as bright as the old slides; the hematoxylin 
part is fine however. I use eosin in 70% EtOH.
Any suggestions as to how I can get the red pulp red? 
Thanks, I like perusing the topics.

Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
Edmond, OK 73034
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