Good advice from Gayle. I have yet to be successful her with GFAP on
frozens and need to come back with an anti -GFP. The most sensitive
antibody I have found is from Roche, cocktail of 2 monoclonal mouse
Ab's. I have not had a problem with mouse on mouse as we are usually
dealing with neuronal tissue. 

Jamie Erickson did a poster around 98 at NSH and will send you her
conclusions. You could look in the archives for her information.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Monfils, Paul [mailto:PMonfils@Lifespan.org] 
Sent: Monday, October 24, 2005 10:25 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] GFP in Paraffin Sections?

Does anyone know if GFP (Green Fluorescent Protein) maintains its
fluorescence through paraffin processing?  Can the fluorescence be
viewed in
an otherwise unstained paraffin section?  After deparaffinization,
should
the section be coverslipped with an aqueous medium, or the standard
medium
used for paraffin sections?

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