I use this almost exclusively as I work with prions as well. If you are
working with new antibodies in a research setting I would experiment
with the times. I think the company is giving guidelines for clinical
applications. I have some antibodies that differ somewhat however in my
experience 30seconds - 5 minutes @120C is adequate for most. I have
found the company to be most helpful with adjusting the settings if
necessary.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: wen eng [mailto:weneng2004@yahoo.com] 
Sent: Thursday, October 27, 2005 10:28 AM
To: histonet
Subject: [Histonet] Decloaking Chamber (high pressure cooker)

Hello,
 
Does anybody use Decloaking Chamber from Biocare Medical? How should I
set the SP1 temperature and time if I need 20 minutes treatment? The
factory setting is 125 degree 30 seconds and the tech support said 20
minutes is too long. She suggested me to keep 30 seconds. I still want
the suggestion from here. How do you set your program?
 
Thanks!
Wen  

		
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