Dear Flavia,

I would ideally use an embedding medium, and section with the specimen
temperature at -10º to -12ºC and the knife temperature at -25ºC or below.



Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Flavia Danielski [mailto:fladanielski@yahoo.com.br]
Sent: 14 October 2005 15:13
To: lista discussao
Subject: [Histonet] 6um pancreas slice


histo-people:
I would be really glad if someone could help me with a
basic protocol for cryosections of pancreas... my goal
is to reach 6um sections... does OCT work in this
case?
Tks
Flavia

Flávia Helena da Silva
Lab. de Imunogenética, Depto. de Genética, UFRGS
Av. Bento Gonçalves, 9500 prédio 43323/lab 212C
Bairro Agronomia.
POA-RS-BRAZIL
phone: 0055-(51)33166737








_______________________________________________________
Promoção Yahoo! Acesso Grátis: a cada hora navegada você acumula cupons e
concorre a mais de 500 prêmios! Participe! http://yahoo.fbiz.com.br/

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet