RE: [Histonet] 4N HCL

From:"Owen, Michael P" (by way of histonet)

Dear Steven,

The information below combined with the recent post should give you some
assistance. Good luck.

Center for Food Safety and Applied Nutrition
Bacteriological Analytical Manual Online
January 2001
Reagents Index

1 N Hydrochloric Acid
HCl (concentrated) 89 ml
Distilled water to make 1 liter

Based on this data, 4 N HCl would be prepared by adding (89 * 4 = 356 mL) of
concentrated HCl to 644 mL distilled water. Remember to add acid to water
for safety.

I highly recommend you place the container that will hold the solution into
an tub of ice or ice water when you combine the acid and water as the
reaction is very exothermic.

Michael P. Owen, Regulatory Microbiologist
U.S. Food and Drug Administration Pacific Regional Lab Northwest
22201 23rd Drive SE Bothell, WA 98021-4421
Phone: 425-483-4865     E-Mail:

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