## [Histonet] spleen sectioning

**From:** | Sharon Cooperman (by way of histonet) |

Dear Histonetites,
I have been asked to prepare some FFPE spleens that are cut "along
the long axis". (Apparently the standard cut for
hematologists/pathologists to use?) The way a spleen looks to me,
there are three possible planes to cut it in; one perpendicular to
the longest dimension and two parallel to the longest dimension. Of
the the two planes parallel to the longest dimension, one would be
parallel to the second longest dimension and one would be
perpendicular to the second longest dimension. I've been cutting my
spleens parallel to the longest dimension and perpendicular to the
second longest dimension, because that's where I seem to get the most
information, and it also looks like what I see in my hematopathology
textbook. Is that correct? I know there's probably a standard way
to do it that everybody knows but I'm not a histologist, pathologist
or hematologist and I'm pretty uninformed about the standard clinical
ways to do spleens. I would appreciate any advice, references, etc.
Thanks in advance!
Sharon
--
Sharon Cooperman
NIH, NICHD, CBMB 301.435-8417
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
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