[Histonet] collagen embeding

From:Niloufar Fozouni

Hello every one,
I need to embed pure collagen in particular substances
in different concentration and stain them (van
gieson's  method).
I've tried embedding collagen in gel and gelatin  as a
mold and then embedding that  in OCT for
cryosectioning but, couldn't get uniform section and
also after staining the collagen washed off.
I need to do that as a  calibration in polarized light
If any one has suggestions please let me know.
Thank you.
Physics Dept.
Oakland University

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